Experimental principle of human non-small cell lung cancer antigen (LTA) ELISA kit
English name: Human nonsmall cell lung cancer / Lung tumor Antigen, LTA ELISA
1. The level of human non-small cell lung cancer antigen (LTA) ELISAKit in specimens was determined by double antibody sandwich method.
2. First coat the microplate with purified human non-small cell lung cancer antigen (LTA) ELISAKit antibody to make a solid phase antibody.
3. Then add non-small cell lung cancer antigen (LTA) ELISAKit to the microwells coated with mAb, and then combine with HRP-labeled non-small cell lung cancer antigen (LTA) ELISAKit antibody to form antibody-antigen-enzyme-labeled antibody complex After thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the non-small cell lung cancer antigen (LTA) ELISAKit in the sample.
5. Finally, use a microplate reader to measure the absorbance (OD value) at a wavelength of 450 nm, and calculate the concentration of human non-small cell lung cancer antigen (LTA) ELISAKit in the sample through the standard curve
.
Objective: To determine the content of non-small cell lung cancer antigen (LTA) ELISAKit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children
Bring your own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
Product Range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit
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