ELISA kits in clinical laboratories generally do not pay much attention to the preparation of reagents. The usual practice is to take the reagents out of the refrigerator during the experiment, and ignore the problem that this practice may affect the later incubation time. The immediate consequence is the false negative test for some weakly positive specimens. Therefore, the preparation of reagents in ELISA measurement is the most critical. Before the experiment, remove the kit from the refrigerator and let it stand at room temperature for more than 20 minutes before performing the measurement, so that the kit is at room temperature before use. Education of Balance Medicine | The purpose of the ELISA kit to do so is mainly to enable the temperature in the reaction microwell to reach the required height quickly in the subsequent incubation reaction step to meet the measurement requirements. Secondly, the plate washing solution in the current commercial ELISA kits needs to be prepared by diluting the provided concentrated solution when it is used in the laboratory. Therefore, the quality of distilled or deionized water used for dilution should be guaranteed. ELISA kit In addition, when the kit uses OPD as a substrate, the substrate solution should be prepared temporarily before the reaction develops color.
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