The globulin enzyme-linked immunoassay indicates that this reagent is for research use only: This kit is used to determine the content of globulin in plant tissues, cells and other related samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of globulin in the specimen. The microporous plate was coated with purified globulin antibody to prepare a solid phase antibody, and globulin was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled globulin. The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with globulin in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentration of globulin in the sample was calculated from a standard curve. This reagent is for research use only: This kit is used to determine the content of globulin in plant tissues, cells and other related samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of globulin in the specimen. The microporous plate was coated with purified globulin antibody to prepare a solid phase antibody, and globulin was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled globulin. The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with globulin in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentration of globulin in the sample was calculated from a standard curve.
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