The principle of the UV-Vis spectrophotometer is:
The ultraviolet-visible absorption spectrum of a molecule is an absorption spectrum generated by the transition of an electron level after absorption of ultraviolet visible radiation by certain groups in the molecule. It is a band spectrum that reflects information about certain groups in the molecule. Qualitative analysis can be performed using standard spectrograms in combination with other means.
According to Lambert-Beer's law: A = εbc, (A is the absorbance, ε is the molar absorptivity, the thickness of the pool, c is the concentration of the solution) can be quantitative analysis of the solution.
You can use the UV-Vis spectrophotometer to determine the maximum and minimum absorption wavelengths of a given pesticide in a solution.
Prepare the solution - under the spectral detection - adjust the detection spectrum range and speed - scan the spectrum - the maximum absorbance at the wavelength is the maximum absorption wavelength, and the wavelength at the minimum absorbance is the minimum absorption wavelength.
The difference between UV-visible spectrophotometer and visible spectrophotometer
The difference between the ultraviolet-visible spectrophotometer and the visible spectrophotometer is that the measurement wavelength range is different. The ultraviolet light is generally used for a hydrogen lamp, and the measurement wavelength ranges from 180 to 350 nm. It can be seen that a tungsten lamp is generally used, and the measurement wavelength range is 320 to 1000 nm. The so-called UV-Vis spectrophotometer means that the instrument can be replaced with a light source and can measure compounds with absorption peaks in the ultraviolet and visible portions.
It is normal to find that the absorbance exceeds 2 and it is no longer displayed.
Absorbance is the negative logarithm of light transmittance. When the absorbance exceeds 2, the light transmittance is less than 1%. Below the detection limit of the instrument, it is no longer displayed.
As for whether or not you can use a spectrophotometer, it depends on the wavelength you are measuring.
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