Abstract: A high-performance capillary electrophoresis amperometric detection method was developed for the determination of aloe content. The influence of various parameters, including buffer type, concentration, pH, working potential, organic modifier, operating voltage, and injection time, on the detection performance was systematically investigated. A borate buffer system composed of Na₂B₄O₇ and NaOH (30 mmol/L Na₂B₄O₇) was selected, with a working potential set at -0.8 V. Methanol (2.5% v/v) was used as the organic modifier to enhance separation efficiency. The analysis was carried out at an applied voltage of 15 kV, with an injection time of 10 seconds. The pH of the buffer solution was adjusted to 9.5 to ensure optimal conditions for the electrochemical detection of aloin. The column-end amperometric detection was performed under alkaline conditions, providing high sensitivity and selectivity. The method exhibited a wide linear range from 0.05 to 50 mg/L, with a linear equation of Y = 856.2 + 123.3c and a correlation coefficient of 0.9994. The detection limit was determined to be 0.01 mg/L, demonstrating the reliability and precision of the proposed method for the quantitative analysis of aloe components. This technique offers a rapid, accurate, and environmentally friendly approach for quality control in aloe-based products.
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