Recently, the Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences and the Shanghai Animal Disease Prevention and Control Center have successfully established pet (canine and cat) rabies, tuberculosis, toxoplasmosis, erythrozoonosis and leptospirosis patients with zoonosis detection technology. It is reported that about three-quarters of newly discovered human infectious diseases are zoonotic diseases. The prevention and control of zoonotic diseases has become a topic of concern to all human beings. Compared with the classic method, Shanghai has successfully established five zoonotic diseases detection methods Simple, fast, high sensitivity and strong specificity; developed a rapid diagnostic kit for Toxoplasma gondii; completed 1 new veterinary drug application; declared 2 national patents, and conducted an epidemiological survey of dogs, cats and zoonoses in Shanghai.
Rabies
Rabies virus can infect all warm-blooded animals, and once pets and people become infected, almost no one is spared. The most important thing to control the disease is prevention. In order to evaluate the immunoprotective efficacy of the vaccine and innovative thinking of researchers, the international first established the ELISA method for detecting the level of neutralizing antibodies in animal serum samples using PCR method, and the coincidence rate with the French Synbiotics ELISA kit is 96%. This method is easy to operate and suitable for the detection of a large number of serum samples. It overcomes the shortcomings of traditional serological methods such as tedious operation, long test period, live poison, and the risk of dispersal. It will provide reliable detection for the monitoring of rabies virus neutralizing antibodies tool.
Toxoplasmosis
Toxoplasmosis is very harmful to humans and animals, leading to premature delivery, miscarriage and fetal developmental malformations in pregnant women. It is one of the main causes of death for patients with immunocompromised patients (such as AIDS patients). The research team established a Nested-PCRRFLP detection technology for Toxoplasma gondii, which can greatly improve the sensitivity and distinguish between Toxoplasma gondii and Neospora; rapid diagnostic reagent for Toxoplasmosis has been prepared, which has strong specificity, high sensitivity and good repeatability. , Fast and simple, without the need for equipment and other advantages, showing excellent performance advantages in the diagnosis of toxoplasmosis and epidemiological investigations at the grassroots level.
Tuberculosis
Tuberculosis is the disease with the highest mortality rate among infectious diseases caused by a single cause. There are about 2 billion people infected with tuberculosis bacteria in the world. The number of tuberculosis patients in China ranks second in the world, and the number of infected people exceeds 400 million. The research team established a fluorescent quantitative PCR detection technology for Mycobacterium tuberculosis in dogs and cats. It has good sensitivity and strong specificity, and the compliance rate with the solid culture method of Mycobacterium tuberculosis ("gold standard") is more than 95%. The diagnosis time will provide a convenient means for the general investigation of tuberculosis in dogs and cats.
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