**Human Scd163 ELISA Kit – For the Quantitative In Vitro Determination of Human Soluble CD163 in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Other Body Fluids**
*For Laboratory Research Use Only. Not for Diagnostic or Therapeutic Procedures.*
This ELISA kit is designed for the accurate and reliable quantification of soluble Cluster of Differentiation 163 (Scd163) in various biological samples. The method is based on the enzyme-linked immunosorbent assay (ELISA) principle, using a competitive binding approach with specific antibodies. The Stop Solution changes the color from blue to yellow, and the intensity of the color is proportional to the concentration of Scd163 in the sample. Calibration standards are included to generate a standard curve, allowing for precise determination of Scd163 levels in unknown samples.
**Intended Use**
The Human Scd163 ELISA Kit is intended for research use only and is not suitable for diagnostic or clinical applications. It is ideal for studying inflammatory responses, immune regulation, and disease progression related to CD163 expression.
**Sample Collection and Storage**
- **Serum**: Collect using a serum separator tube. Allow clotting for 2 hours at room temperature or overnight at 4°C before centrifuging at 2000×g for 20 minutes. Remove serum and assay immediately, or aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
- **Plasma**: Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g (2–8°C). Store at -20°C and avoid freeze-thaw cycles.
- **Cell Culture Supernatants, Tissue Homogenates, and Other Biological Fluids**: Centrifuge to remove particulates and assay immediately or store at -20°C. Thaw only once.
**Materials Required but Not Supplied**
- 37°C incubator
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes, disposable tips, and absorbent paper
- Distilled or deionized water
**Reagents Provided**
All reagents should be stored at 2–8°C. Check the expiration date on the label before use.
- Microtiter Strip Plate (12×8 strips / 12×4 strips)
- Standard (6 vials, 0.5 ml/vial)
- Sample Diluent (6.0 ml / 3.0 ml)
- HRP-Conjugate Reagent (10.0 ml / 5.0 ml)
- 20X Wash Solution (25 ml / 15 ml)
- Chromogen Solution A (6.0 ml / 3.0 ml)
- Chromogen Solution B (6.0 ml / 3.0 ml)
- Stop Solution (6.0 ml / 3.0 ml)
- Closure Plate Membrane (2 units)
- User Manual (1 copy)
- Sealed Bags (1 unit)
**Precautions**
- Do not mix reagents from different kit lots.
- Bring all reagents and samples to room temperature (20–25°C) before use.
- Do not use beyond the expiration date.
- Keep microtiter plates in their sealed bags until needed.
- Wear gloves when handling biological samples.
- Dispose of waste properly after inactivation for at least 30 minutes.
- Handle chromogen solutions carefully; avoid contact with heat or flame.
**Reagent Preparation**
- Wash Solution (1X): Dilute 1 volume of 20X Wash Solution with 19 volumes of distilled or deionized water. Store at 2–8°C for up to one month.
**Assay Procedure**
1. Prepare all reagents before starting. Run standards and samples in duplicate.
2. Add 50 µL of standard or sample to each well (blank well receives no addition).
3. Add 100 µL of HRP-conjugate reagent to all wells except the blank. Cover with adhesive strip and incubate for 60 minutes at 37°C.
4. Wash the plate 4 times manually or automatically.
5. Add 50 µL of Chromogen A and 50 µL of Chromogen B to each well. Incubate for 15 minutes at 37°C, protected from light.
6. Add 50 µL of Stop Solution to each well. The color will change from blue to yellow.
7. Measure OD at 450 nm. Calculate mean OD values for each standard and sample. Subtract blank OD from all readings. Plot a standard curve and determine Scd163 concentrations.
**Performance Characteristics**
- Sensitivity: <1.0 ng/mL
- Assay Range: 5 ng/mL – 160 ng/mL
- Intra-assay CV (%): <15%
- Inter-assay CV (%): <15%
- No cross-reactivity or interference observed
**Storage**
- Store unused reagents at 2–8°C for frequent use, or at -20°C for long-term storage.
- Ensure all components are used before the expiration date.
**Note:** Always read the entire user manual before performing the assay. Follow all safety and disposal guidelines. This product is not intended for human consumption or medical diagnosis.
coffe table, central table, console table, marble metal finished wooden veneer, luxury minimalist exquisite ,aesthetic space elevated
Foshan Beausimpe Furniture Co., Ltd , https://www.minottiitaly.com