Muloon et al. Prepared the artificial antigen SAL-BSA of salinomycin, and immunized BALB / c mice with it, and screened out 16 monoclonal antibodies. On this basis, a Ci-ELISA method for detecting salinomycin was established. The monoclonal antibody has high selectivity to salinomycin, can recognize methyl salinomycin without cross-reacting with lasalicin and monensin. The study also compared the two methods of HPLC and ELISA. The results show that the two methods have a good correlation (P <0.001), but the ELISA method is more sensitive, and its detection limit (50ug / kg) is higher than that of HPLC (100ug / kg) low. Mount et al. Established an immunoassay for monensin, with a detection limit of 2ng / ML. Shen Jianzhong et al. Synthesized 5 maduramycin artificial antigens by mixed anhydride method and active ester method, and prepared rabbit anti-maduramycin antiserum by immunizing rabbits with M-KLH. The working concentration of antiserum was 1: 1.6X10 (3 ) -1: 2.56X10 (4), and on this basis, a Ci-ELISA method for the detection of maduramycin was established, using M-C6-OVA as the coating source, and the IC50 value of the standard curve of maduramycin was 30.1 -32.3ng / ML, the slope is 2.20-2.55 (medium is PBS-10% methanol), cross-reaction data shows that the antibody has a high selectivity for the characteristic structure of Maduramycin.
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