At 3 p.m. yesterday, my manager, He, received a call from Manager Li of the technical department. During the conversation, Manager Li shared some important observations from this month’s customer consultations regarding the Elisa test kits. He mentioned that several key points are often overlooked by users, and he had compiled them into a detailed summary to send directly to our email. He encouraged us to review the information and share it with customers to help improve their experimental outcomes. Below is the latest technical summary provided by Manager Li from Shanghai Hengyuan Technology for August:
1. Always read the instructions carefully and confirm that the kit is within its valid period before starting the experiment.
2. Ensure all reagents and necessary equipment listed in the instructions are complete, including items like test tubes, pipette tips, pipettes, and centrifuges.
3. Standardize specimen preparation by preparing enough volume for 2–3 replicates. If the samples cannot be tested immediately, store them properly using cryopreservation methods.
4. Allow all reagents to reach room temperature before use, as per the manufacturer's guidelines.
5. When selecting antibodies, it's best to pair a monoclonal antibody with a polyclonal one. If using two monoclonals, make sure they recognize different epitopes. It’s also recommended to choose antibody pairs from the same supplier for better compatibility.
6. To determine the linear range of the antigen’s standard curve, perform a 2-fold serial dilution from 2000 pg/ml down to 15 pg/ml. You can enhance sensitivity by following the standard ELISA protocol, using amplification kits, third-party reagents, or adjusting the enzyme substrate system.
Shanghai Hengyuan updates its website weekly with the latest experimental techniques and industry insights. If you're looking for high-quality ELISA kits, feel free to contact our sales team at Shanghai Hengyuan Biological Company. Our kits come with full technical support and offer a free testing service to ensure optimal performance. Let us help you achieve accurate and reliable results in your experiments.
Ombre Lace Front Wig,Hair Extensions 3 Tone,Hair Bundles For Women,Body Wave Hair
Xuchang KANAZI Hair Products Co., Ltd. , https://www.kanazihair.com