ELISA kit composition and operating precautions

1. Kit composition

1

30 times concentrated washing solution

20ml × 1 bottle

7

Stop solution

6ml × 1 bottle

2

Enzyme reagent

6ml × 1 bottle

8

Standard product (480μg / L)

0.5ml × 1 bottle

3

Enzyme coated plate

12 holes × 8

9

Standard dilution

1.5ml × 1 bottle

4

Sample diluent

6ml × 1 bottle

10

Instructions

1 serving

5

Developer A liquid

6ml × 1 bottle

11

Sealing film

2 sheets

6

Developer B liquid

6ml × 1 / bottle

12

sealed bag

1

2. Operation steps

Dilution of standard products: This kit provides one original standard product. The user can perform dilution in a small test tube according to the following chart.

240μg / L

Standard No. 5

150μl of original standard is added to 150μl standard dilution

120μg / L

Standard 4

150μl Standard No. 5 is added to 150μl standard dilution

60μg / L

Standard 3

150μl Standard No. 4 is added to 150μl Standard Diluent

30μg / L

Standard No. 2

150μl Standard No. 3 is added to 150μl Standard Diluent

15μg / L

Standard 1

150μl Standard No. 2 is added to 150μl Standard Diluent

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